Microbiological and Biochemical Activity in Soil Contaminated with Pyrene Subjected to Bioaugmentation

Abstract The aim of the study was to determine efficacy bioaugmentation in pyrene-contaminated soil based on microbial counts, colony development index (CD), ecophysiological diversity (EP), enzyme activity, and an assay residual pyrene levels soil. samples were contaminated with doses 100 1000 mg kg −1 DM Two bacterial consortia used study: P1 ( Bacillus frigoritolerans Z2B-19, simplex 2–134, thuringiensis ex4) P2 pumilus Bp-11, safensis L22, aerophilus KUDC1741). following parameters determined: counts organotrophic bacteria, actinobacteria, fungi; CD; EP; activity enzymes. degradation also established. Microbiological influenced by level contamination pyrene, test time, type consortium. Pyrene had a stimulatory effect diversifier CD values, EP Bioaugmentation initially promoted growth microorganisms, but ultimately diminished microorganisms for accelerated removal from soil, 24.6% 16.4% case consortium, respectively. use provides favorable conditions effective elimination As microbiological biochemical properties improved initial phase study, this method can be recommended bioremediation soils.